Towards hybrid one-pot/one-electrode Pd-NPs-based nanoreactors for modular biocatalysis
نویسندگان
چکیده
Here, fundamental aspects affecting template-assisted engineering of oxidase-associated peroxide oxidation co-catalysis the modeled microanalytical system based on hybrid palladium nanoparticles (Pd-NPs) with tailored functional properties were studied. By an accurate tuning and validation experimental setup, a modular Pd-NPs-doped one-pot/one-electrode amperometric nanobiosensor for advanced multiplex analyte detection was constructed. The specific operational conditions (electrochemical read-out mode, pH, regeneration procedure) allowed reliable sensing L-lactate (with linear dynamic range, LDR = 500 µM – 2 mM, R2 0.977), D-glucose 200 50 0.987), hydrogen 20 100 0.998) glutaraldehyde 1 0.971). In addition, mechanistic influencing performance studied in detail. designed showed thin layer electrochemical behavior that greatly enhanced electron transfer between electrode. Finally, procedure algorithm towards its usage biocatalysis developed. reported strategy can readily be considered as guideline fabrication commercialized nanobiosensors biocatalysis.
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ژورنال
عنوان ژورنال: Biochemical Engineering Journal
سال: 2021
ISSN: ['1873-295X', '1369-703X']
DOI: https://doi.org/10.1016/j.bej.2021.108132